Method and device for automated removal of cells and/or cell colonies

US9822331B2 · kind B2 · utility

Assignee

• ALS Automated Lab Solutions GmbH · DE

Inventors

• Rafael Backhaus
• Jens Eberhardt · Hermsdorf, DE
• Marco Meinhard · Eckernförde, DE
• Gerd Bornmann · Weimar, DE

Key dates

• Filing date: Sep 20, 2007
• Grant date: Nov 21, 2017
• Expiry date: Feb 21, 2031

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12M41/48
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The method comprises an automated removal of cells and/or cell colonies from a cell culture whilst executing a first detection step for selecting cells and/or cell colonies with reference to corporeal and/or physical parameters and detecting position data and storing the detected position data of the selected cells and/or cell colonies in a position database.In order to be able to select special cells and/or cell colonies having special properties from the detected cells and/or cell colonies, at least one second detection step for detecting at least one further parameter of the cells and/or cell colonies is then executed, comparative data from the data of the first and second detection step are created, cells and/or cell colonies are selected with reference to the comparative data and the position data from the position database are transferred to a harvesting unit.