Production of metabolites
US9822374B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | May 24, 2011 |
| Grant date | Nov 21, 2017 |
| Priority date | — |
| Expiry date | May 24, 2031 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P7/22
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A recombinant micro-organism such as Saccharomyces cerevisiae which produces and excretes into culture medium a stilbenoid metabolite product when grown under stilbenoid production conditions, which expresses in above native levels a ABC transporter which transports said stilbenoid out of said micro-organism cells to the culture medium. The genome of the Saccharomyces cerevisiae produces an auxotrophic phenotype which is compensated by a plasmid which also expresses one or more of said enzymes constituting said metabolic pathway producing said stilbenoid, an expression product of the plasmid is genetically modified to include a ubiquitination tag sequence. Expression of an enzyme participating in catabolism of phenylalanine by the Ehrlich pathway is optionally reduced compared to its native expression level.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.