High-throughput mutagenized cell screening system for selective single cell extraction
US9822390B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Oct 28, 2013 |
| Grant date | Nov 21, 2017 |
| Priority date | — |
| Expiry date | Dec 26, 2034 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01L2400/086
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The subject invention pertains to a microfluidic apparatus and methods for screening and isolating a target cell from a population of cells. The apparatus comprises a first microfluidic layer comprising microfluidic channels; a second microfluidic layer comprising microfluidic channels; and a microfluidic cell analysis layer comprising a top hanging blocking structure located directly below each location where the first layer microfluidic channels overlap with the second layer microfluidic channels and a cell trap juxtaposed to each of the top hanging blocking structures. The top hanging blocking structures can close or open the juxtaposed cell trap when either or both the first or second layer microfluidic channels located directly above the top hanging blocking structure are sufficiently pressurized and/or sufficiently depressurized. The methods for screening and isolating a target cell from a population of cells comprise screening the population of cells using the apparatus and isolating the target cell interest therefrom.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.