Compositions, methods and systems for polymerase chain reaction assays

US9822393B2 · kind B2 · utility

Assignee

• Bio-Rad Laboratories, Inc. · US

Inventors

• Adam Lowe · Tracy, US
• Geoff McDermott · Pleasanton, US
• Christopher Hindson · Pleasanton, US
• Erin R. Chia · Walnut Creek, US
• Amy L. Hiddessen · Tracy, US
• Benjamin Hindson · Pleasanton, US
• Chunxiao Han · Dublin, US
• Yaqi Wang · San Jose, US

Key dates

• Filing date: Mar 7, 2014
• Grant date: Nov 21, 2017
• Expiry date: Jul 29, 2034

Classification

• Technology area (CPC B): Performing Operations; Transporting
• CPC primary: B01L3/502784
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present disclosure provides methods, devices, systems and compositions for detecting nucleic acids in polymerase chain reaction assays, such as droplet digital polymerase chain reaction (ddPCR) assays. The present disclosure provides methods, devices, systems and compositions for detecting nucleic acids in ddPCR assays using intercalating dyes. A dual surfactant system with at least one fluorosurfactant and at least one non-ionic non-fluorosurfactant may be employed for droplet generation and nucleic acid detection.