Phage-based bacterial detection assay

US9828625B2 · kind B2 · utility

Assignee

• Phage Diagnostics, Inc. · US

Inventors

• Michael Sandor Koeris · Natick, US
• Julie Thomason · Reading, US
• Michael Cappillino · Reading, US
• Robert Patrick Shivers · Watertown, US
• Daniel Robert Brownell · Arlington, US
• Jayson Bowers · Cambridge, US
• Timothy Kuan-Ta Lu · Boston, US
• Edyta Krzymanska-Olejnik · Brookline, US

Key dates

• Filing date: Jun 19, 2014
• Grant date: Nov 28, 2017
• Expiry date: Jun 19, 2034

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2333/255
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Methods of detecting target bacteria are provided. In some embodiments the methods comprise exposing the sample to a phage capable of infecting a set of target bacteria and comprising a heterologous nucleic acid sequence encoding a marker. In some embodiments the target bacteria comprise Listeria. In some embodiments the target bacteria are all Listeria. Recombinant Listeria phage comprising a heterologous nucleic acid sequence encoding a marker are also provided as are useful combinations of such phage and articles of manufacture comprising such phage, among other things.