Patent · US Active

Methods of enhancing genetic diagnosis

US9828630B2 · kind B2 · utility

0Cited by

0References

10Claims

0Family size

Assignee

BioLamina AB · SE

Inventors

Karl Tryggvason · Stockholm, SE
Kristian Tryggvason · Sundbyberg, SE

Key dates

Filing date	Nov 30, 2012
Grant date	Nov 28, 2017
Priority date	—
Expiry date	Feb 20, 2034

Classification

Technology area (CPC G)Physics
CPC primaryG01N2800/38
WIPO fieldMeasurement
WIPO sectorInstruments

Abstract

The present disclosure relates to the use of laminin-521. Laminin-521 can maintain stem cells in vitro pluripotency, enable self-renewal, and enable single cell survival of human embryonic stem cells. When pluripotent human embryonic stem cells are cultured on plates coated with recombinant laminin-521 (laminin-11), in the absence of differentiation inhibitors or feeder cells, the embryonic stem cells proliferate and maintain their pluripotency. Methods of enhancing genetic diagnosis by expanding a single stem cell on laminin-521 prior to PCR amplification are disclosed herein.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.