Single nucleotide detection method
US9828631B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Apr 9, 2014 |
| Grant date | Nov 28, 2017 |
| Priority date | — |
| Expiry date | Apr 24, 2034 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01L3/502784
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for determining the sequence of nucleotide bases in a polynucleotide analyte is provided. It is characterized by the steps of (1) generating a stream of single nucleotide bases from the analyte by pyrophosphorolysis; (2) producing captured molecules by reacting each single nucleotide base with a capture system labelled with detectable elements in an undetectable state; (3) releasing the detectable elements from each captured molecule in a detectable state and (4) detecting the detectable elements so released and determining the sequence of nucleotide bases therefrom. The method can be used advantageously in sequencers involving the use of microdroplets.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.