Methods and means for metabolic engineering and improved product formation by micro-organisms
US9834773B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Feb 14, 2007 |
| Grant date | Dec 5, 2017 |
| Priority date | — |
| Expiry date | Sep 23, 2031 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N1/38
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Described are methods and means for metabolic engineering and improved product formation by a filamentous micro-organism or a low G+C gram-positive bacterium. Disclosed is that DasR and DasR binding sites play an important and universal role in the control of gene expression in micro-organisms. Based on this finding, provided are multiple useful applications, such as a method for regulating the expression of a gene of interest, a method for controlling metabolism, a method for decreasing undesired expression and many more. Moreover, provided are means that can be used to establish said methods: for example a micro-organism in which the DasR binding site in operable linkage with a particular gene has been modified to obtain increased or decreased expression of a protein (being a desired or undesired protein) encoded by said gene.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.