Methods for detecting nucleic acid sequence variants
US9834817B2 · kind B2 · utility
1Cited by
5References
30Claims
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Key dates
| Filing date | Mar 15, 2013 |
| Grant date | Dec 5, 2017 |
| Priority date | — |
| Expiry date | Oct 25, 2033 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2600/112
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides methods for detecting the presence or absence of a nucleic acid variant in a target region. These methods include amplifying the target region with a forward primer and a reverse primer in the presence of a selector blocker. The selector blocker includes a sequence complementary to the target region in the absence of the nucleic acid variant. The methods further include detecting amplification of the target region where amplification of the target region indicates the presence of the nucleic acid variant in the target region. The nucleic acid variant can include deletions, mutations or insertions.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.