Method and system of microfluidic immunoassay using magnetic beads

US9835623B2 · kind B2 · utility

Assignee

• Caliper Life Sciences, Inc. · US

Inventors

• Andrew Barry · Chelmsford, US
• Laurel Provencher · Hopkinton, US
• Seth Cohen · Westford, US
• I-Jane Chen · Ridgewood, US
• Jun Yan · Franklin, US
• Jingjing Wang · Shanghai, CN

Key dates

• Filing date: Jan 15, 2015
• Grant date: Dec 5, 2017
• Expiry date: Dec 1, 2035

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/54326
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A microfluidic Western blot method and system including a microfluidic western blot method for immunoassay of proteins, the method including introducing a sample including the proteins onto a chip; electrophoretically separating the proteins; binding the separated proteins to beads to form protein-attached beads, the beads being magnetic; flowing the protein-attached beads into a magnetic holding region; applying a magnetic field to the magnetic holding region to fix the protein-attached beads in place within the magnetic holding region; binding primary antibodies to target proteins on the protein-attached beads; binding secondary antibodies to the bound primary antibodies; and detecting the bound secondary antibodies.