Combined biomarker measurement of fibrosis
US9835631B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Feb 3, 2016 |
| Grant date | Dec 5, 2017 |
| Priority date | — |
| Expiry date | Feb 3, 2036 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2800/7052
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Provided herein is a sandwich immunoassay for detecting cross-linked PIIINP that has at least two strands of PIIINP joined together by inter-strand cross-linking each having a C-terminal neo-epitope of PIIINP that is generated by N-protease cleavage of intact type III procollagen. A biological sample having the cross-linked PIIINP is contacted with a first surface-bound monoclonal antibody and then by a second monoclonal antibody, both specifically reactive with a neoepitope in the C-terminal sequence of PIIINP, and then binding of the second monoclonal antibody is determined. Also provided is a method for evaluating the efficacy of an antagonist drug targeting lysyl oxidases via the immunoassay and a kit containing a solid support binding the first monoclonal antibody and containing the second monoclonal antibody.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.