Light-switchable gene expression system

US9839698B2 · kind B2 · utility

Assignee

• East China University of Science and Technology · CN

Inventors

• Yi Yang · Shanghai, CN
• Xue Wang · Shanghai, CN
• Xianjun Chen · Shanghai, CN

Key dates

• Filing date: Feb 27, 2012
• Grant date: Dec 12, 2017
• Expiry date: Feb 27, 2032

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2830/001
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Provided is a light-switchable gene expression system, comprising: a) a recombinant light-switchable transcription factor-encoding gene, said recombinant light-switchable transcription factor comprising a first polypeptide as the DNA bonding domain, a second polypeptide as the photosensitive domain, and a third polypeptide as the transcription regulatory domain; b) a target transcription unit comprising at least one reaction element recognized and bound by the first polypeptide, a promoter regulated by the third polypeptide and a nucleotide sequence to be transcribed. Also provided is an eukaryotic expression vector comprising said light-switchable gene expression system, and a method for regulating gene expression in a host cell by using the light-switchable gene expression system. Also provided is a reagent kit containing different elements of the light-switchable gene expression system. The light-switchable gene expression system has a quick, effective and powerful induction with little or no toxicity. It is safer than other inducers, and can spatiotemporally control gene expression.