CRISPR-based compositions and methods of use

US9840702B2 · kind B2 · utility

Assignee

• INTEGRATED DNA TECHNOLOGIES, INC. · US

Inventors

• Michael Allen Collingwood · North Liberty, US
• Ashley Mae Jacobi · Tiffin, US
• Garrett Richard Rettig · Coralville, US
• Mollie Sue Schubert · Cedar Rapids, US
• Mark Aaron Behlke · Coralville, US

Key dates

• Filing date: Dec 18, 2015
• Grant date: Dec 12, 2017
• Expiry date: Dec 18, 2035

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2320/53
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

This invention pertains to modified compositions for use in CRISPR systems, and their methods of use. In particular, length-modified and chemically-modified forms of crRNA and tracrRNA are described for use as a reconstituted guide RNA for interaction with Cas9 of CRIPSR systems. The resultant length-modified and chemically-modified forms of crRNA and tracrRNA are economical to produce and can be tailored to have unique properties relevant to their biochemical and biological activity in the context of the CRIPSR Cas9 endonuclease system.