Method for preparing in situ-formed hydrogel using enzyme-immobilized support, and biomedical use thereof
US9844610B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Sep 11, 2013 |
| Grant date | Dec 19, 2017 |
| Priority date | — |
| Expiry date | Jan 27, 2034 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61L2400/06
- WIPO fieldMedical technology
- WIPO sectorInstruments
Abstract
The present invention relates to a method for preparing a hydrogel, including the steps of: preparing an enzyme-immobilized support; making contacting with a polymer having a phenol or aniline functional group at a side change and the support in the presence of hydrogen peroxide to cross-link the same; and obtaining a hydrogel by separating the enzyme-immobilized support. According to the present invention, a hydrogel is formed by cross-linking a polymer having a phenol or aniline functional group at a side chain through an enzyme-immobilized support, and thus a hydrogel containing no enzymes is obtained. Therefore, the biomedical application range of a hydrogel can be expanded since it is possible to overcome an in vivo safety problem such as immune reaction caused by an enzyme.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.