Membrane-integrated microfluidic device for imaging cells

US9844779B2 · kind B2 · utility

Assignee

• The Charles Stark Draper Laboratory, Inc. · US

Inventors

• Jeffrey T. Borenstein · Holliston, US
• Joseph L. Charest · Cambridge, US
• Joseph Cuiffi · State College, US
• Alla Epshteyn · Brookline, US
• Angela B. Holton · Tampa, US
• James C. Hsiao · Watertown, US

Key dates

• Filing date: Jan 13, 2012
• Grant date: Dec 19, 2017
• Expiry date: Jul 6, 2033

Classification

• Technology area (CPC B): Performing Operations; Transporting
• CPC primary: B01L2300/0887
• WIPO field: Chemical engineering
• WIPO sector: Chemistry

Abstract

A microfluidic device is provided. The microfluidic device includes a first transparent, solid support layer. A first polymeric layer defining at least one chamber is attached to the first transparent, solid support layer. A semi-permeable membrane is attached to the first polymeric layer. A second polymeric layer is attached to the opposite side of the semi-permeable membrane from the first polymeric layer. The second polymeric layer has a thickness of less than 300 microns and defines at least one chamber positioned to overlap with at least one chamber in the first polymeric layer. A first manifold structure is attached to an input end of at least one chamber and a second manifold structure is attached to an output end of at least one chamber.