Maintenance of genomic stability in cultured stem cells
US9845457B2 · kind B2 · utility
11Cited by
136References
8Claims
0Family size
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Key dates
| Filing date | Dec 28, 2015 |
| Grant date | Dec 19, 2017 |
| Priority date | — |
| Expiry date | Dec 28, 2035 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2501/998
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present application relates to methods and compositions for the generation of therapeutic cells having reduced incidence of karyotypic abnormalities. In several embodiments cardiac stem cells are cultured in an antioxidant-supplemented media that reduces levels of reactive oxygen species, but does not down regulate DNA repair mechanisms. In several embodiments, physiological oxygen concentrations are used during culture in order to increase the proliferation of stem cells, decrease the senescence of the cells, decrease genomic instability, and/or augment the functionality of such cells for cellular therapies.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.