Attenuated live vaccine against mycoplasmal pneumonia of swine (MPS) and use thereof

US9849168B2 · kind B2 · utility

Assignee

• Jiangsu Academy of Agricultural Sciences · CN

Inventors

• Guoqinq Shao · Nanjing, CN
• Qiyan Xiong · Nanjing, CN
• Maojun Liu · Nanjing, CN
• Zhixin Feng · Nanjing, CN
• Yanna Wei · Nanjing, CN
• Haiyan Wang · Cranbury, US
• Fangfang Bai · Nanjing, CN
• Yuan Gan · Nanjing, CN
• Li-Ting Wang · Meekrap-oord, CN
• Daohua Zhang · Nanjing, CN
• Dongxia Liu · Nanjing, CN
• Lizhong Hua · Nanjing, CN
• Yuzi Wu · Nanjing, CN
• Yun Bai · Beijing, CN
• Zhanwei Wang · Beijing, CN

Key dates

• Filing date: Jan 26, 2014
• Grant date: Dec 26, 2017
• Expiry date: Jan 26, 2034

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12R2001/35
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Disclosed are an attenuated live vaccine against mycoplasmal pneumonia of swine (MPS) and use thereof. In the present invention, pathological lung tissues of swine having typical Mycoplasma hyopneumoniae (Mhp) infection and no obvious other pathogenic infections are screened, and subcultured 100 generations in lungs of newborn rabbits; then, Mhp strains are isolated and serially subcultured in a medium; and the Mhp strain AN306 is obtained by screening a plurality of strains, which is deposited with an accession number: CCTCC NO. M2012431. Also disclosed is a live vaccine formulation against MPS prepared on the basis of the attenuated strain and comprising live attenuated strain, a pharmaceutically acceptable carrier or excipient, and optionally an adjuvant and immunogens of other pathogens.