Generation and use of polyclonal and monoclonal antibodies specific for 3-phosphohistidine

US9850319B2 · kind B2 · utility

Assignees

• Salk Institute for Biological Studies · US
• Sanofi · FR

Inventors

• Tony Hunter · Village of La Jolla, US
• Stephen Rush Fuhs · Village of La Jolla, US
• Jill Meisenhelder · Village of La Jolla, US
• Jacques Mauger · Tucson, US
• Magda Stankova · Tucson, US
• Fahad Al-Obeidi · Tucson, US
• Robert A. Binne · Tucson, US

Key dates

• Filing date: Jul 1, 2015
• Grant date: Dec 26, 2017
• Expiry date: Jul 1, 2035

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2317/565
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Isolated monoclonal antibodies and or antigen binding fragments thereof are disclosed herein that specifically bind polypeptides comprising a histidine phosphorylated at N3 (3-pHis). Nucleic acids encoding these antibodies, vectors including these nucleic acids, and host cells transformed with these vectors and nucleic acids are also disclosed. Methods are also disclosed for using these antibodies, such as for detection of polypeptides comprising a histidine phosphorylated at N3 (3-pHis). In some embodiments, the methods can be used to investigate signal transduction pathways.