Patent · US Active

Method for increasing the secretion of recombinant proteins

US9850513B2 · kind B2 · utility

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0References

21Claims

0Family size

Assignee

GREENOVATION BIOTECH GMBH · DE

Inventors

Wolfgang Jost · Glückstadt, DE
Mathias Knappenberger · Laupheim, DE
Doreen Claussnitzer · Munich, DE
Andreas Schaaf · Mittenaar, DE

Key dates

Filing date	Apr 17, 2013
Grant date	Dec 26, 2017
Priority date	—
Expiry date	Apr 17, 2033

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N2500/60
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The invention relates to a method for producing a recombinant protein in cells with a cell wall, comprising the step of increasing the secretion of the recombinant protein through the cell wall by expression of the protein in the cells in a culture medium containing a combination of a surface-active polymer and monovalent metal ions and with an osmolarity at least 0.32 osmol/L, said invention further relating to culture media and nutrient mixtures for the method.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.