Compact automated cell counter

US9850517B2 · kind B2 · utility

Assignee

• Bio-Rad Laboratories, Inc. · US

Inventors

• Tom McCollum · Berkeley, US
• Paul Patt · Danville, US
• Frank Shen · Martinez, US
• Daniel Y. Chu · San Francisco, US
• Don Flory · Hercules, US
• Mike Griffin · San Jose, US
• Xin Heng · Hercules, US
• Eli A. Hefner · Fairfield, US

Key dates

• Filing date: Sep 27, 2013
• Grant date: Dec 26, 2017
• Expiry date: May 7, 2035

Classification

• Technology area (CPC G): Physics
• CPC primary: G02B21/34
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Biological cells in a liquid suspension are counted in an automated cell counter that focuses an image of the suspension on a digital imaging sensor that contains at least 4,000,000 pixels each having an area of 2×2 μm or less and that images a field of view of at least 3 mm2. The sensor enables the counter to compress the optical components into an optical path of less than 20 cm in height when arranged vertically with no changes in direction of the optical path as a whole, and the entire instrument has a footprint of less than 300 cm2. Activation of the light source, automated focusing of the sensor image, and digital cell counting are all initiated by the simple insertion of the sample holder into the instrument. The suspension is placed in a sample chamber in the form of a slide that is shaped to ensure proper orientation of the slide in the cell counter.