Ultra-high-sensitive assay of protein and nucleic acid and kit, and novel enzyme substrate
US9851309B2 · kind B2 · utility
1Cited by
2References
5Claims
0Family size
Assignees
Inventor
Key dates
| Filing date | Mar 23, 2012 |
| Grant date | Dec 26, 2017 |
| Priority date | — |
| Expiry date | Jun 13, 2033 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/924
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Provided is a ultra-high-sensitivity assay in which the assay can be made on a commonly used assay apparatus such as an absorptiometer and a plate reader or with naked eyes. The high-sensitivity assay in which the assay can be made on a commonly used assay apparatus or with naked eyes can be provided by combining an enzyme cycling method using thio-NAD(P) as a coenzyme, a labeling enzyme and a substrate for the labeling enzyme optimally, and by amplifying thio-NAD(P)H, which is a signaling substance, exponentially and then quantifying the thio-NAD(P)H colorimetrically.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.