Methods and devices for high fidelity polynucleotide synthesis
US9856471B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jun 27, 2014 |
| Grant date | Jan 2, 2018 |
| Priority date | — |
| Expiry date | Dec 25, 2034 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01L2400/0439
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed are methods for synthesizing and/or assembling at least one polynucleotide product having a predefined sequence from a plurality of different oligonucleotides. In exemplary embodiments, the methods involve synthesis and/or amplification of different oligonucleotides immobilized on a solid support, release of synthesized/amplified oligonucleotides in solution to form droplets, recognition and removal of error-containing oligonucleotides, moving or combining two droplets to allow hybridization and/or ligation between two different oligonucleotides, and further chain extension reaction following hybridization and/or ligation to hierarchically generate desired length of polynucleotide products.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.