Specific oligonucleotide aptamer for the identification of T-2 toxin

US9856482B2 · kind B2 · utility

Assignee

• Jiangnan University · CN

Inventors

• Zhouping Wang · Beijing, CN
• Xiujuan Chen · Wuxi, CN
• Huajie Gu
• Yu XIA · Longbeilingcun, CN
• Shijia Wu · Wuxi, CN
• Nuo Duan · Wuxi, CN
• Xiaoyuan MA · Wuxi, CN

Key dates

• Filing date: May 26, 2015
• Grant date: Jan 2, 2018
• Expiry date: May 26, 2035

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12R2001/645
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

This invention provides a small molecule oligonucleotide aptamer binding to T-2 toxin specifically, and the sequence of the ssDNA aptamer is SEQ ID NO: 1. Through the graphene oxide separation-based Systematic Evolution of Ligands by Exponential Enrichment, single-stranded oligonucleotide aptamer with high affinity and specific identification of T-2 toxin was obtained in vitro selection. The aptamer has a broad application prospect, which can be used for separation and enrichment of trace T-2 toxin in the sample. It can also be used as a reporter aptamer for the detection of T-2 toxin in food by means of functional groups labeling and other means.