Plant transformation with in vivo assembly of a trait

US9856484B2 · kind B2 · utility

Assignee

• Bayer CropScience N.V. · BE

Inventors

• Anatoly Giritch · Halle (Saale), DE
• Sylvestre Marillonnet · Bodenwerder, DE
• Victor Klimyuk · Leipzig, DE
• Yuri Gleba · Berlin, DE

Key dates

• Filing date: Jan 30, 2004
• Grant date: Jan 2, 2018
• Expiry date: Jun 8, 2027

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/8205
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A process of endowing a plant or plant cells with a trait of interest by expressing an RNA sequence of interest, said process comprising: providing plant cells or cells of said plant with a first vector and a second vector and selecting cells endowed with said trait of interest, wherein said first vector contains a first nucleotide sequence with a first segment coding, in 5′ to 3′ direction, for—a 5′ part of said RNA sequence of interest and—a 5′ part of an intron; and said second vector contains a second nucleotide sequence with a second segment coding, in 5′ to 3′ direction, for—a 3′ part of an intron and—a 3′ part of said RNA sequence of interest.