Methods of nucleic acid fractionation and detection
US9856514B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 18, 2014 |
| Grant date | Jan 2, 2018 |
| Priority date | — |
| Expiry date | Feb 12, 2036 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/101
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention provides methods of detecting a nucleic acid present in a biological sample, comprising combining the biological sample with a lysis buffer to form a lysis mixture comprising nucleic acid released from cells in said biological sample; and subjecting a volume of the lysis mixture to size-exclusion chromatography in a column comprising a volume of size-exclusion medium. In certain embodiments, the lysis buffer separates double-stranded nucleic acid into single-stranded nucleic acid. In certain embodiments, the elution can have a flow rate of separation of less than 10 minutes to produce an eluted solution comprising isolated nucleic acid. The invention provides for a method of accurately and rapidly detecting products of nucleic acid amplification.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.