Method and apparatus for biomolecule analysis
US9857368B2 · kind B2 · utility
Inventor
Key dates
| Filing date | Jan 30, 2017 |
| Grant date | Jan 2, 2018 |
| Priority date | — |
| Expiry date | Jan 30, 2037 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2001/4088
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Disclosed are devices or apparatus and methods for membrane-, bead- or cell-based biomolecule analysis, where a detectable signal can be measured after affinity binding and/or enzymatic reaction occurs on the membranes, beads or cells. The apparatus or device can rapidly separate the beads or cells from the liquid solution without using centrifugation, vacuum, or magnetic force. The apparatus or device includes a sample well for receiving a sample containing cells or beads, as well as other aqueous solutions, a porous filter membrane at the bottom of the well which is capable of retaining the cells or beads on its upper surface by size exclusion, and an absorbing plug in touch with the filter membrane for removing the liquid solution. Multiple devices can be arranged in a multi-well array in a plate so that signals from multiple assays can be directly analyzed in a plate reader or an imaging instrument.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.