Patent · US Active

Method for the detection of cytosine methylations in DNA

US9863001B2 · kind B2 · utility

0Cited by

8References

16Claims

0Family size

Assignee

EPIGENOMICS AG · DE

Inventors

Reimo Tetzner · Berlin, DE
Jurgen Distler · Berlin, DE

Key dates

Filing date	Aug 13, 2004
Grant date	Jan 9, 2018
Priority date	—
Expiry date	Sep 20, 2027

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Q2600/154
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The invention relates to a method for analyzing cytosine methylations in DNA sequences, according to which non-methylated cytosines are first converted into uracil while 5-methylcytosine remains unmodified. The DNA is then amplified by means of a polymerase and at least one primer whose 5 end is connected to a probe via a linker. The probe is intramolecularly hybridized onto the amplified products in accordance with the methylation state of the DNA, hybridization being detectable via different detection systems. The inventive method is particularly suitable for diagnosing and predicting cancer diseases and other diseases associated with a modification of the methylation state as well as for predicting undesired effects of medicaments.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.