Molecular assay reagents and methods
US9863004B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 2, 2012 |
| Grant date | Jan 9, 2018 |
| Priority date | — |
| Expiry date | Nov 2, 2032 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2600/16
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods, kits, and compositions for evaluating the quality of nucleic acids within a biological sample for analysis in a molecular assay are provided. A kit is provided for evaluating a biological sample containing potentially degraded DNA, comprising a control reagent and a target amplification reagent, wherein the control amplification reagent comprises one or more pairs of amplification oligonucleotides capable of amplifying a medium chain acyl-coenzyme A dehydrogenase (MCAD) nucleic acid molecule or a complement thereof, and wherein the target amplification reagent comprises one or more pairs of amplification oligonucleotides capable of amplifying a non-MCAD target gene of interest or a complement thereof. Frequently, one or more of the amplification oligonucleotides is/are labeled.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.