Method for bisulfite treatment

US9868756B2 · kind B2 · utility

Assignee

• EPIGENOMICS AG · DE

Inventors

• Christine Markert-Hahn · Penzberg, DE
• Dirk Block · Bichl, DE

Key dates

• Filing date: Aug 24, 2015
• Grant date: Jan 16, 2018
• Expiry date: Aug 24, 2035

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6834
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present application is directed to a method for performing a bisulfite reaction to determine methylation positions in a nucleic acid, i.e. methylated and non-methylated cytosines, whereby the nucleic acid is bound to a solid phase during the deamination and/or desulfonation step of the bisulfite reaction. The solid phase is preferably a material comprising glass or silica, more preferably a glass fleece, glass membrane or a magnetic glass particle. Further, the use of a solid phase for binding a nucleic acid during the deamination and/or desulfonation step of the bisulfite reaction is disclosed and a kit containing a bisulfite reagent and a solid phase.