Patent · US Active

Method for improving GlcNAc production of recombinant bacillus subtilis

US9868970B2 · kind B2 · utility

1Cited by

1References

12Claims

0Family size

Assignee

Jiangnan University · CN

Inventors

Long Liu · Wuxi, CN
Jian Chen · Wuxi, CN
Guocheng Du · Wuxi, CN
Jianghua Li · Wuxi, CN
Yang Gu · Wuxi, CN
Yang Song · Kunming, CN
Jieying Deng · Wuxi, CN
Yawen ZHAO · Beijing, CN

Key dates

Filing date	Jul 8, 2016
Grant date	Jan 16, 2018
Priority date	—
Expiry date	Oct 10, 2036

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Y604/01001
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The invention provides an effective method for improving N-acetylglucosamine (GlcNAc) production by engineered B. subtilis Deletion of phosphoenolpyruvate carboxykinase encoding gene pckA and encoding pyruvate kinase gene pyK in recombinant GlcNAc-producing strain BSGNK-PxylA-glmS-P43-GNA1 (BSGNK) is first performed to enhance GlcNAc production, followed by overexpression of pyruvate carboxylase encoding gene pycA for facilitating cell growth. Finally, the GlcNAc production of the recombinant strain BPTS3 reached to 11.3 g/L, which was 1.84-fold of BSGNK. This method can be used for improve cellular property of engineered B. subtilis for GlcNAc production, which can be further applied to industrial production of GlcNAc.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.