Methods and apparatus for sequential amplification reactions
US9873909B2 · kind B2 · utility
1Cited by
56References
6Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Nov 25, 2014 |
| Grant date | Jan 23, 2018 |
| Priority date | — |
| Expiry date | May 24, 2035 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01L2400/0644
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention provides methods and apparatus for carrying out multiple amplification reactions in a single reaction chamber by successive cycles of loading reaction mixture, amplifying, and removing spent reaction mixture in a fluidly closed reaction system. In particular, the present invention allows amplification of a plurality of target polynucleotides from a single sample by carrying out under closed-loop control successive amplifications of different target polynucleotides from different portions of the sample.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.