Methods, systems, and devices for detecting and identifying microorganisms in microbiological culture samples
US9874555B2 · kind B2 · utility
Assignee
Inventors
- Kristin Weidemaier
- Robert L. Campbell
- Erin Gooch Carruthers
- Adam C. Curry
- Kevin G. Dolan
- Andrea Liebmann-Vinson
- Wendy Woodley
- Melody M. H. Kuroda
- Eric A. Fallows
- Miroslaw Bartkowiak
- Scott N. Danhof
- Gregory S. Kramer
- Thomas D. Haubert
- Michael L. Marshall
- James A. Prescott
- Randy J. Somerville
- M. Scott Ulrich
- David S. Sebba
Key dates
| Filing date | Mar 15, 2013 |
| Grant date | Jan 23, 2018 |
| Priority date | — |
| Expiry date | Aug 19, 2033 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB03C2201/26
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Provided herein are methods, systems, and devices for detecting and/or identifying one or more specific microorganisms in a culture sample. Indicator particles, such as surface enhanced Raman spectroscopy (SERS)-active nanoparticles, each having associated therewith one or more specific binding members having an affinity for the one or more microorganisms of interest, can form a complex with specific microorganisms in the culture sample. Further, agitating magnetic capture particles also having associated therewith one or more specific binding members having an affinity for the one or more microorganisms of interest can be used to capture the microorganism-indicator particle complex and concentrate the complex in a localized area of an assay vessel for subsequent detection and identification. The complex can be dispersed, pelleted, and redispersed so that the culture sample can be retested a number of times during incubation so as to allow for real-time monitoring of the culture sample.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.