Systems and methods for cryopreservation of cells
US9877475B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jan 8, 2015 |
| Grant date | Jan 30, 2018 |
| Priority date | — |
| Expiry date | Mar 28, 2035 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA01N1/147
- WIPO fieldBasic materials chemistry
- WIPO sectorChemistry
Abstract
An auto-nucleating device includes a tube containing a crystalline cholesterol matrix. The ends of the tube are closed by a membrane that is impermeable to the cholesterol but permeable to liquids contained in a cryopreservation vessel. The auto-nucleating device provides a site for ice nucleation during freezing of the liquid within the vessel. One such cryopreservation vessel is a flexible vial having a closed port at one adapted to be pierced by a needle to withdraw the liquid within, and an opposite end that is initially open to receive the liquid. Another vessel includes an adaptor mounted to liquid container with a tubular branch closed by a needle septum and another tubular branch provided with a barbed fitting for engaging a flexible tube that terminates in a needle septum. In another embodiment, the vessel includes an inlet and vent branch at the top of the container and an outlet septum at a bottom opening.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.