Chromogenic glucuronidase substrates and uses
US9879303B2 · kind B2 · utility
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2References
9Claims
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Key dates
| Filing date | Nov 3, 2014 |
| Grant date | Jan 30, 2018 |
| Priority date | — |
| Expiry date | Nov 3, 2034 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/942
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Chromogenic substrates for β-D-glucuronidase activity comprising monoglucuronides of some 1,2-dihydroxyaromatic derivatives. When cleaved these form soluble colored conjugates with multivalent metal ions such as iron ions. The substrates may be used in conjunction with chromogenic substrates for other enzymes in microbial detection and identification especially involving liquid media. Microbes can be grown in the presence of the substrates and the compounds providing the metal ion. The substrates are particularly useful for detecting β-D-glucuronidase-positive E. coli. Synthetic methods for making the compounds are described.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.