Methods and compositions for nucleic acid sample preparation

US9879318B2 · kind B2 · utility

Assignee

• PACIFIC BIOSCIENCES OF CALIFORNIA, INC. · US

Inventors

• Igor D. Vilfan · East Palo Alto, US
• Stephen Turner · Menlo Park, US

Key dates

• Filing date: Sep 4, 2014
• Grant date: Jan 30, 2018
• Expiry date: Nov 9, 2035

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2535/122
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention provides methods and compositions useful for supplying high throughput nucleic acid sequencing systems with templates. The methods circumvent the need for costly, labor-intensive cloning and cell culture methods and can be scaled to accommodate template production for a variety of sequencing applications, e.g., sequencing individuals' genomes, sequencing subpopulations of transcripts from a gene of interest, and/or gene expression profiling. Particularly preferred embodiments of the methods vastly improve the preparation of cDNA from mRNA samples, e.g., by randomizing errors introduced during the process, thereby allowing these errors to be readily distinguished from true variants present in the mRNA samples.