Detection of target nucleic acid sequences using dual-labeled immobilized probes on solid phase
US9885081B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Oct 20, 2011 |
| Grant date | Feb 6, 2018 |
| Priority date | — |
| Expiry date | Nov 25, 2031 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6837
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to a novel method for detection of target nucleic acid sequences on a solid phase using dual-labeled immobilized probes and its resistance to a 5′ to 3′ exonuclease activity of a DNA polymerase. Because the label is remained on the solid substrate by resistance to nucleases due to labeling of a base component the internal nucleotide, the present invention requires no consideration of a suitability of position of the label for remaining on the solid substrate. The present invention ensures to minimize background signal by positioning labels at a site on probes suitable to maximize quenching efficiency of the dual label system, since it permits to freely determine the position of the internal label on probes.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.