Chemical crosslinkers
US9889203B2 · kind B2 · utility
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8Claims
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Key dates
| Filing date | Mar 14, 2014 |
| Grant date | Feb 13, 2018 |
| Priority date | — |
| Expiry date | Jun 6, 2034 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y302/0102
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Disclosed herein are methods of chemical conjugation comprising contacting a lysosomal enzyme with a first crosslinking agent to introduce aldehyde groups; contacting a lysosomal targeting peptide with a second crosslinking agent to introduce a hydrazide group at the N-terminal residue; contacting the lysosomal enzyme with aldehyde groups of step a. with the lysosomal targeting peptide with a hydrazide group at the N-terminal residue of step b; and forming a lysosomal enzyme-lysosomal targeting peptide conjugate.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.