Isolated oligonucleotide and use thereof in nucleic acid sequencing

US9890375B2 · kind B2 · utility

Assignee

• BGI SHENZHEN · CN

Inventors

• Chunyu Geng · Nanhu, CN
• Dennis Ballinger · Menlo Park, US
• Yanyan Zhang · Lo Wu, CN
• Shujin Fu · Nanhu, CN
• Lingyu He · Nanhu, CN
• Wenwei Zhang · Longbeilingcun, CN
• Hui Jiang · Lo Wu, CN

Key dates

• Filing date: Sep 12, 2014
• Grant date: Feb 13, 2018
• Expiry date: Sep 12, 2034

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2565/537
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Provided are an isolated oligonucleotide and a use thereof in nucleic acid sequencing, wherein the isolated oligonucleotide comprises a first strand, wherein the 5′-end nucleotide of the first strand has a phosphate group, and the 3′-end nucleotide of the first strand is a dideoxynucleotide, and a second strand, wherein the 5′-end nucleotide of the second strand does not have a phosphate group, and the 3′-end nucleotide of the second strand is a dideoxynucleotide, wherein the first strand is longer than the second strand in length, and a double-stranded structure is formed between the first strand and the second strand.