Acquisition of fragment ion mass spectra of ions separated by their mobility

US9891194B2 · kind B2 · utility

Assignees

• Bruker Daltonik GmbH · DE
• Matthias Mann · DE

Inventors

• Matthias Mann · Munich, DE
• Oliver Räther · Lilienthal, DE
• Melvin Andrew Park · Billerica, US
• Markus Lubeck · Bremen, DE

Key dates

• Filing date: Nov 3, 2015
• Grant date: Feb 13, 2018
• Expiry date: Nov 3, 2035

Classification

• Technology area (CPC H): Electricity
• CPC primary: H01J49/4215
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The invention provides a method for acquiring fragment ion spectra of substances in complex substance mixtures wherein a trapped ion mobility spectrometer (“TIMS”) is used as the ion mobility separator separation device. The fragment ion spectra may be used for the identification of high numbers of proteins in complex mixtures, or for a safe quantification of some substances, by their fragment ion mass spectra in a mass spectrometer with up-front substance separator. TIMS with parallel accumulation provides the unique possibility to prolong the ion accumulation duration to find more detectable ion species without decreasing the measuring capacity for fragment ion mass spectra. The high measurement capacity for fragment ion mass spectra permits the repeated measurement of low abundance ion species to improve the quality of the fragment ion spectra.