SWATH# data-independent acquisition technology for the detection of host cell protein contaminants in biotherapeutics protein products
US9891203B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Jun 3, 2014 |
| Grant date | Feb 13, 2018 |
| Priority date | — |
| Expiry date | Jun 3, 2034 |
Classification
- Technology area (CPC H)Electricity
- CPC primaryH01J49/004
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Systems and methods are provided for detecting host cell contaminants in a protein biotherapeutic product using sequential windowed acquisition tandem mass spectrometry. Sequential windowed acquisition is performed on a protein biotherapeutic product sample by sequentially stepping a precursor mass window across a mass range, fragmenting transmitted precursor ions of each stepped precursor mass window, and analyzing product ions produced from the fragmented transmitted precursor ions. The sequential windowed acquisition is performed without any information about contaminating proteins before data acquisition, and produces data for every product ion of every transmitted precursor ion for the mass range. One or more measured product ion spectra are received, and compared to a library of host cell proteins. One or more host cell contaminants are detected by reporting host cell proteins from the library that match the one or more measured product ion spectra.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.