Patent · US Active

Mutant with enhanced secretion of L-asparaginase and its application

US9896678B2 · kind B2 · utility

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0References

2Claims

0Family size

Assignee

Jiangnan University · CN

Inventors

Song Liu · San Jose, US
Yue Feng · Wuxi, CN
Jian Chen · Wuxi, CN
Guocheng Du · Wuxi, CN
Yun Jiao · Wuxi, CN
Jie Ruan · Wuxi, CN
Hongye Cheng · Wuxi, CN
Hui Gao · Suzhou, CN

Key dates

Filing date	Jun 22, 2016
Grant date	Feb 20, 2018
Priority date	—
Expiry date	Aug 1, 2036

Classification

Technology area (CPC A)Human Necessities
CPC primaryA61K38/00
WIPO fieldPharmaceuticals
WIPO sectorChemistry

Abstract

The present invention provides a mutant with enhanced secretion of L-asparaginase and its application, which relates to the field of enzyme engineering. This invention successfully constructed a mutant with enhanced secretion of L-asparaginase through N-terminal deletion on amino acid sequence of L-asparaginase, and then expressed the mutant via a recombinant strain. Compared with the wild L-asparaginase, the secretion ability of L-asparaginase mutant in present invention significantly improves by 3.14 times compared to that of wild type. The recombinant strain that constructed in this prevention has an L-asparaginase yield of 407.6 U/mL and a production efficiency of 9.26 U/(mL/h), which reaches to the highest yield been reported.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.