Fused NHase with improved specific activity and stability
US9896679B2 · kind B2 · utility
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Key dates
| Filing date | Sep 30, 2015 |
| Grant date | Feb 20, 2018 |
| Priority date | — |
| Expiry date | Nov 5, 2035 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y402/01084
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides a fused NHase with improved specific activity and stability, which relates to the field of genetic engineering. This invention provides a method of overexpressing a fused NHase in E. coli and producing a mutant NHase with improved stability and product tolerance. The invention provides a simple, efficient and safe method of making mutant NHase, and can produce a large amount of soluble NHases in a short period. The present invention makes a contribution to large-scale industrial production and further theoretical study of NHases.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.