Imaging individual mRNA molecules using multiple singly labeled probes

US9896720B2 · kind B2 · utility

Assignee

• Rutgers, the State University of New Jersey · US

Inventors

• Arjun Raj · Lansdowne, US
• Sanjay Tyagi · New York, US

Key dates

• Filing date: Sep 10, 2009
• Grant date: Feb 20, 2018
• Expiry date: Jan 20, 2030

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6841
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A method for probing a target sequence of messenger ribonucleic acid molecules (mRNA's) in a fixed, permeabilized cell, said target sequence including at least 30 non-overlapping probe binding regions of 15-100 nucleotides, comprising immersing said cell in an excess of at least 30 nucleic acid hybridization probes, each singly labeled with the same fluorescent label and each containing a nucleic acid sequence that is complementary to a different probe binding region of said target sequence; washing said fixed cell to remove unbound probes; and detecting fluorescence from said probes.