Real-time, label-free detection of macromolecules in droplets based on electrical measurements
US9896725B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | May 11, 2015 |
| Grant date | Feb 20, 2018 |
| Priority date | — |
| Expiry date | Feb 2, 2036 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/48707
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for detecting presence of a macromolecule of interest in a test droplet. A set of detection electrodes are provided in contact with a fluidic channel. The test droplet is provided in vicinity of the detection electrodes through the fluidic channel. An alternate current (AC) power at a first frequency is applied across the set of detection electrodes. A first measurement value that reflects electrical characteristics (e.g., impedance) of the test droplet at the first frequency is obtained. This value is compared with a corresponding reference value, wherein the corresponding reference value is obtained by measuring a reference droplet containing the macromolecule of interest or free of the macromolecule at the first frequency. The presence of the macromolecule in the test droplet is thus determined based on the comparison.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.