Methods and compositions for discrimination between cytosine and modifications thereof, and for methylome analysis
US9896726B2 · kind B2 · utility
7Cited by
1References
15Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jul 22, 2015 |
| Grant date | Feb 20, 2018 |
| Priority date | — |
| Expiry date | Jul 22, 2035 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y305/04005
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Compositions and methods are provided for discrimination between cytosine and modifications thereof using cytidine deaminases and/or oxygenases. Variants of wild type cytidine deaminases are described which show reduced bias with respect to adjacent nucleotides upstream of the cytosine. The methods provide a rapid and convenient use of enzymes to obtain methylomes.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.