Nanodiamond counting
US9897603B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Jul 18, 2017 |
| Grant date | Feb 20, 2018 |
| Priority date | — |
| Expiry date | Jul 18, 2037 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/543
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Methods and systems for detecting the locations of individual instances of an analyte (e.g., individual cells, individual molecules) in an environment are provided. The environment includes functionalized fluorophores that are configured to selective interact with (e.g., bind with) the analyte and that have a fluorescent property that can be modulated (e.g., a fluorescence intensity that can be affected by the presence of a magnetic field). Detecting the location of individual instances of the analyte includes illuminating the environment and detecting signals emitted from the fluorophores in response to the illumination during first and second periods of time. Detecting the location of individual instances of the analyte further includes modulating the modulatable fluorescent property of the fluorophores during the second period of time and determining which individual fluorophores in the environment are bound to the analyte based on the signals detected during the first and second periods of time.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.