Systems for processing multiple assays background
US9903003B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 15, 2013 |
| Grant date | Feb 27, 2018 |
| Priority date | — |
| Expiry date | Mar 15, 2033 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01L2400/0487
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods, devices, and systems for performing polymerase chain reaction (PCR) amplification and melt data acquisition according to a single slug approach in which a single slug in a microfluidic channel fills an entire thermal zone of the microfluidic channel, and the thermal zone used for both PCR temperature cycling and melt data acquisition. A detector may be configured to detect fluorescence from the thermal zone during the PCR temperature cycling for real-time PCR and/or during temperature ramping in the melt data acquisition. Slug position control may be achieved by detecting leading or trailing edges in a slug build target zone into which a slug passes after passing through the thermal zone. The single slug approach may break coupling between one or more events of the PCR amplification and melt data acquisition and enable events to be independently optimized.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.