Patent · US Active

Method for identifying aptamers

US9903859B2 · kind B2 · utility

0Cited by

8References

19Claims

0Family size

Assignee

Albert-Ludwigs-Universitaet Freiburg · DE

Inventors

Guenter Roth · Glückstadt, DE
Christine Reinemann · Leipzig, DE
Beate Strehlitz · Leipzig, DE

Key dates

Filing date	Oct 25, 2012
Grant date	Feb 27, 2018
Priority date	—
Expiry date	Jan 19, 2033

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Q1/6811
WIPO fieldMeasurement
WIPO sectorInstruments

Abstract

The invention relates to a method for identifying aptamers, having the following steps —bringing a mixture of oligonucleotides into contact with an aptamer target structure and binding at least some of the oligonucleotides to the target structure, —separating the oligonucleotides which have been bound to the aptamer target structure from the aptamer target structure and from oligonucleotides that are not bound to the aptamer target structure, —amplifying individual oligonucleotides which were bound to the aptamer target structure in a physically separate manner and producing a plurality of physically separate amplicons, each amplicon predominantly containing one type of oligonucleotides, —specifying a specific marker for a plurality of the physically separate amplicons such that each of the marked amplicons can be uniquely identified using the specified marker of the amplicon, —sequencing oligonucleotides in a plurality of marked amplicons and assigning the marker that is specific for the amplicon to the sequence of the type of oligonucleotides in the amplicon for each amplicon examined by means of the sequencing process —analyzing the binding properties of the types of oligonucleo…

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.