Patent · US Active

Enhanced itaconic acid production in Aspergillus with increased LaeA expression

US9909152B2 · kind B2 · utility

0Cited by
2References
16Claims
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Inventors

Key dates

Filing dateOct 30, 2015
Grant dateMar 6, 2018
Priority date—
Expiry dateFeb 9, 2036

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12Y204/01258
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

Fungi, such as Aspergillus niger, having a dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase (Alg3) gene genetic inactivation, increased expression of a loss of aflR expression A (LaeA), or both, are described. In some examples, such mutants have several phenotypes, including an increased production of citric acid relative to the parental strain. Methods of using the disclosed fungi to make citric acid are also described, as are compositions and kits including the disclosed fungi. Further described are Aspergillus terreus fungi overexpressing the LaeA gene and the use of such fungi for the production of itaconic acid.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.