Highly selective nucleic acid amplification primers

US9909159B2 · kind B2 · utility

Assignee

• Rutgers, the State University of New Jersey · US

Inventors

• Salvatore A. E. Marras · Roselle Park, US
• Diana Vargas-Gold · Millburn, US
• Sanjay Tyagi · New York, US
• Fred R. Kramer · Yonkers, US

Key dates

• Filing date: Feb 7, 2014
• Grant date: Mar 6, 2018
• Expiry date: Nov 4, 2034

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/686
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

This invention discloses multi-part primers for primer-dependent nucleic acid amplification methods. Also disclosed are primer-dependent nucleic acid amplification reactions, particularly DNA amplification reactions, reaction mixtures and reagent kits for such reactions. This invention relates to primer-dependent nucleic acid amplification reactions, particularly DNA amplification reactions such as PCR, and primers, reaction mixtures and reagent kits for such reactions and assays employing same.